Antitumor activity without on-target off-tumor toxicity of GD2–chimeric antigen receptor T cells in patients with neuroblastoma. Karin Straathof, Rebecca Wallace, Neha Jain, Thalia Loka, Sarita Depani, Gary Wright, Simon Thomas, Gordon Weng-Kit Cheung, Talia Gileadi, Sian Stafford, Evangelia Kokalaki, Jack Barton, Clare Marriott, Dyanne Rampling, Olumide Ogunbiyi, Ayse U. Akarca, Teresa Marafioti, Sarah Inglott, Kimberly Gilmour, Muhammad Al-Hajj, William Day, Kieran McHugh, Lorenzo Biassoni, Natalie Sizer, Claire Barton, David Edwards, Ilaria Dragoni, Julie Silvester, Karen Dyer, Stephanie Traub, Lily Elson, Sue Brook, Nigel Westwood, Lesley Robson, Ami Bedi, Karen Howe, Ailish Barry, Catriona Duncan, Giuseppe Barone, Martin Pule and John Anderson. Science Translational Medicine  25 Nov 2020:
Vol. 12, Issue 571, eabd6169. DOI: 10.1126/scitranslmed.abd6169

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A primer set for the rapid isolation of scFv fragments against cell surface antigens from immunised rats. Francesco Nannini  Farhaan Parekh , Patrycja Wawrzyniecka , Leila Mekkaoui , Matteo Righi , Fatemeh V Dastjerdi , Jenny Yeung , Claire Roddie , Yuchen Bai , Biao Ma , Mathieu Ferrari , Shimobi Onuoha , Kerry Chester , Martin Pule.  PMID: 33154441  PMCID: PMC7644676 DOI: 10.1038/s41598-020-76069-3

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AUTO6NG overcomes immune suppressive mechanisms in the TME and demonstrate preclinical anti-tumor activity in GD2-expressing solid tumors. Margarida Neves, Lukas Stanczuk, Alireza Alavi, Meggan Czapiga, Rebecca Moore, Frederick Vargas and Muhammad A. Al-Hajj. DOI: 10.1158/1538-7445.AM2020-2661 Published August 2020

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Enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR. Sara Ghorashian, Anne Marijn Kramer, Shimobi Onuoha, Gary Wright, Jack Bartram, Rachel Richardson, Sarah J. Albon, Joan Casanovas-Company, Fernanda Castro, Bilyana Popova, Krystle Villanueva, Jenny Yeung, Winston Vetharoy, Aleks Guvenel, Patrycja A. Wawrzyniecka , Leila Mekkaoui , Gordon Weng-Kit Cheung, Danielle Pinner, Jan Chu, Giovanna Lucchini, Juliana Silva, Oana Ciocarlie, Arina Lazareva, Sarah Inglott, Kimberly C. Gilmour, Gulrukh Ahsan, Mathieu Ferrari , Somayya Manzoor, Kim Champion, Tony Brooks, Andre Lopes, Allan Hackshaw, Farzin Farzaneh, Robert Chiesa, Kanchan Rao, Denise Bonney, Sujith Samarasinghe, Nicholas Goulden, Ajay Vora, Paul Veys, Rachael Hough, Robert Wynn, Martin A. Pule and Persis J. Amrolia

Nature Medicine volume 25, pages1408–1414(2019) describing enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR.

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Phase 1 First-in-Human Study of AUTO2, the First Chimeric Antigen Receptor (CAR) T Cell Targeting APRIL for Patients with Relapsed/Refractory Multiple Myeloma (RRMM). Rakesh Popat, Sonja Zweegman, MD PhD, Jim Cavet, FRCPath, Kwee Yong, Lydia Lee, Jim Faulkner, Ekaterini Kotsopoulou, Muhammad Al-Hajj, Simon Thomas, PhD, Shaun P Cordoba, PhD, Martin Pule, MD, Virginie Cerec, PhD, Vijay G R Peddareddigari, Nushmia Z Khokhar, MD, Tobias F Menne, MD PhD FRCPath. Blood (2019) 134 (Supplement_1): 3112.

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A Rapamycin-Activated Caspase 9-Based Suicide Gene. Maria Stavrou, Brian Philip, Charlotte Traynor-White, Christopher G. Davis, Shimobi Onuoha, Shaun Cordoba, Simon Thomas and Martin Pule
Published:March 08, 2018DOI:https://doi.org/10.1016/j.ymthe.2018.03.001

Publication of article in Molecular Therapy describing small molecule-activated off switch which is designed to allow for the selective elimination of T cells programmed to incorporate Caspase 9 (rapaCasp9) using rapamycin (sirolimus, Rapamune®)

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A Dominant Negative SHP-2 Which Abrogates PD-1 Signalling Pathways and Restores Function of Cytotoxic CAR T Cells. Vania Baldan, PhD, Priyanka Ghongane, MSc, Evangelia Kokalaki, PhD, Wen Chean Lim, PhD, Shimobi Onuoha, PhD, Shaun Paul Cordoba, PhD, Simon Thomas, PhD and Martin Pule, MD PhD. Blood (2017) 130 (Supplement 1): 3190.

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Targeting T-cell receptor β-constant for immunotherapy of T-cell malignancies. Paul M Maciocia, Patrycja A Wawrzyniecka, Brian Philip, Ida Ricciardelli, Ayse U Akarca, Shimobi C Onuoha, Mateusz Legut, David K Cole, Andrew K Sewell, Giuseppe Gritti, Joan Somja, Miguel A Piris, Karl S Peggs, David C Linch, Teresa Marafioti & Martin A Pule. Nature Medicine 2017 Nov 23; 416–1423 (2017);

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A highly compact epitope-based marker/suicide gene for easier and safer T-cell therapy. Philip B, Kokalaki E, Mekkaoui L, Thomas S, Straathof K, Flutter B, Marin V, Marafioti T, Chakraverty R, Linch D, Quezada SA, Peggs KS, Pule M. Blood. 2014 Aug 21;124(8):1277-87.

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